{"product_id":"recombinant-human-myeloperoxidase-mpo-protein-his-tag-pdmh100014","title":"Recombinant Human Myeloperoxidase\/MPO Protein (His Tag) - PDMH100014","description":"\u003cp\u003eRecombinant Human Myeloperoxidase\/MPO Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PDMH100014-20, PDMH100014-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Myeloperoxidase; MPO\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Myeloperoxidase; MPO\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P05164\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cardiovascular; Cancer; immunology; metabolism\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-10His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P05164\u003c\/p\u003e\n\n\u003cp\u003eBackground: Myeloperoxidase (MPO) is a heme-containing\u003cbr\u003e\nenzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that\u003cbr\u003e\ncatalyzes the hydrogen peroxide-dependent\u003cbr\u003e\nconversion of chloride, bromide, and iodide to multiple reactive species. Post-translational\u003cbr\u003e\nprocessing of MPO involves\u003cbr\u003e\nthe insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked\u003cbr\u003e\ndimer composed of a\u003cbr\u003e\n60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond\u003cbr\u003e\nbetween the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion of\u003cbr\u003e\nthe signal sequence and part of the propeptide. Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the\u003cbr\u003e\nformation of 3-chlorotyrosine\u003cbr\u003e\nand dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levels\u003cbr\u003e\nof NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic\u003cbr\u003e\ngranules. Upon cellular activation, it is deposited into pathogen-containing\u003cbr\u003e\nphagosomes.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala49-Ser745\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 80.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 85-95 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43083609014451,"sku":"PDMH100014-20","price":502.6,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43083609047219,"sku":"PDMH100014-100","price":838.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_a7d8cf4d-49bf-4e8d-8678-bae69fb32d51.png?v=1708945169","url":"https:\/\/afsbio.com\/products\/recombinant-human-myeloperoxidase-mpo-protein-his-tag-pdmh100014","provider":"AFSBio Inc.","version":"1.0","type":"link"}