{"product_id":"recombinant-human-nectin-3-pvrl3-protein-his-tagactive-pksh031388","title":"Recombinant Human Nectin-3\/PVRL3 Protein (His Tag)(Active) - PKSH031388","description":"\u003cp\u003eRecombinant Human Nectin-3\/PVRL3 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031388-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Nectin-3; PVRL3\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Poliovirus Receptor-Related Protein 3; CDw113; Nectin-3; CD113; PVRL3; PRR3; PVRR3\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Microbiology; immunology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_056295.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3\/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3\/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2\/Nectin-2 at Sertoli-spermatid junctions. PVRL3\/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized recombinant human PVRL3 at 1 μg\/ml (100 μl\/well) can bind biotinylated Nectin-1 with a linear range of 6. 4-800 ng\/ml. 2. Immobilized recombinant human PVRL3 at 1 μg\/ml (100 μl\/well) can bind Nectin-1\/ Fc chimera with a linear range of 0.156-5 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Asp 400\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 98 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 39.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 65-75 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104310493363,"sku":"PKSH031388-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_9ba00198-acb8-4e75-9b0a-e24517b130e5.png?v=1709294018","url":"https:\/\/afsbio.com\/products\/recombinant-human-nectin-3-pvrl3-protein-his-tagactive-pksh031388","provider":"AFSBio Inc.","version":"1.0","type":"link"}