{"product_id":"recombinant-human-neutrophil-cytosol-factor-1-ncf1-protein-his-tag-pksh032805","title":"Recombinant Human Neutrophil Cytosol Factor 1\/NCF1 Protein (His Tag) - PKSH032805","description":"\u003cp\u003eRecombinant Human Neutrophil Cytosol Factor 1\/NCF1 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032805-10, PKSH032805-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: NCF1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: NCF-1; 47 kDa autosomal chronic granulomatous disease protein\/47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2\/SH3 and PX domain-containing protein 1A; p47-phox\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P14598\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cancer; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P14598\u003c\/p\u003e\n\n\u003cp\u003eBackground: Neutrophil cytosol factor 1( NCF1) is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is characterized as a multicomponent enzyme which is activated to produce superoxide anion. NCF2, NCF1, and a membrane bound cytochrome b558 are required for the activation of the latent NADPH oxidase. The human NCF1 gene encodes a 390 amino acids protein without a signal peptide. The NCF1 gene interacts with other subunits of nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) and plays an important role in innate immunity, producing reactive oxygen species and reducing the severity and duration of parasitic infection and autoimmune disease. NCF1 also has a role in T cell activation.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Val390\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris 100mM NaCl, 1mM DTT, pH8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 45.6 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 45-50 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43068732342451,"sku":"PKSH032805-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43068732375219,"sku":"PKSH032805-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_04751ed9-c445-41cf-9707-c76bb8b5666e.png?v=1708597214","url":"https:\/\/afsbio.com\/products\/recombinant-human-neutrophil-cytosol-factor-1-ncf1-protein-his-tag-pksh032805","provider":"AFSBio Inc.","version":"1.0","type":"link"}