{"product_id":"recombinant-human-noggin-nog-protein-aa-1-232-fc-tagactive-pksh031721","title":"Recombinant Human Noggin\/NOG Protein (aa 1-232, Fc Tag)(Active) - PKSH031721","description":"Recombinant Human Noggin\/NOG Protein (aa 1-232, Fc Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSH031721-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: Noggin; NOG\n\nTarget Symptom: Noggin; SYM1; SYNS1\n\nResearch Area: Developmental Biology; Stem cells\n\nTarget Species: Human\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture\n\nFusion Tag: C-hFc\n\nAccession: NP_005441.1\n\nBackground: Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.\n\nActivity: 1. Measured by its ability to inhibit BMP2-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1. 5-2.0 μg \/mL in the presence of 0.25-0.5 μg\/mL of BMP-2. \n2. Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.1-0.6 μg\/mL in the presence of 50 ng\/mL of hBMP4. \n\nSequence: Met 1-Cys 232\n\nPurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 49.8 kDa\n\nObserved MW: 58-62 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104323633331,"sku":"PKSH031721-100","price":789.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_0e420153-2d2e-4138-b9a4-bc85a9bd10b4.png?v=1709294301","url":"https:\/\/afsbio.com\/products\/recombinant-human-noggin-nog-protein-aa-1-232-fc-tagactive-pksh031721","provider":"AFSBio Inc.","version":"1.0","type":"link"}