{"product_id":"recombinant-human-noggin-nog-protein-rp01237","title":"Recombinant Human Noggin\/NOG Protein - RP01237","description":"\u003cp\u003eRecombinant Human Noggin\/NOG Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01237-10, RP01237-20, RP01237-50, RP01237-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Noggin\/NOG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln28-Cys232) of human Noggin (Accession #NP_005441.1) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: NOG, SYM1, SYNS1, SYNS1A, noggin\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q13253\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 9241\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human BMP2 at 1 μg\/mL (100 μL\/well) can bind Noggin with a linear range of 2-57 ng\/mL.|2. Measured by its binding ability in a functional ELISA.Immobilized Human BMP4 at 0.5 μg\/mL (100 μL\/well) can bind Noggin with a linear range of 4-47 ng\/mL.|3.Measured by its binding ability in a functional ELISA. Immobilized Human Noggin at 1 μg\/mL (100 μL\/well) can bind Noggin Rabbit pAb with a linear range of 1-3.5 ng\/mL.|4. Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 13.28-53.12 ng\/mL in the presence of 50 ng\/mL of recombinant human BMP-4, corresponding to a specific activity of 1.88×10^4 -7.53×10^4 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198344290483,"sku":"RP01237-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198344323251,"sku":"RP01237-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198344356019,"sku":"RP01237-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198344388787,"sku":"RP01237-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_95c8daea-7211-41ce-b82c-ef14ac5c2a37.png?v=1766960841","url":"https:\/\/afsbio.com\/products\/recombinant-human-noggin-nog-protein-rp01237","provider":"AFSBio Inc.","version":"1.0","type":"link"}