{"product_id":"recombinant-human-noggin-rhunoggin-pr1103","title":"Recombinant Human NOGGIN (rHuNOGGIN ) - PR1103","description":"\u003cp\u003eRecombinant Human NOGGIN (rHuNOGGIN )\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR1103-5, PR1103-20\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5µg, 20µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Approximately 46.2 kDa non-disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;95% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: The ED50 was determined by its ability to inhibit 5.0 ng\/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 µg\/ml of NOGGIN, corresponding to a Specific Activity of ＞1.25 x 104 IU\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLt VLRWRCQRRGGQRCG WIPIQYPIIS ECKCSC\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/mg of rHu NOGGIN as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"5µg","offer_id":42882227699891,"sku":"PR1103-5","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"20µg","offer_id":42882227732659,"sku":"PR1103-20","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c343a5df-6d1a-4697-b632-912c483bcf4b.jpg?v=1702049661","url":"https:\/\/afsbio.com\/products\/recombinant-human-noggin-rhunoggin-pr1103","provider":"AFSBio Inc.","version":"1.0","type":"link"}