{"product_id":"recombinant-human-pdgf-aa-protein-active-pksh032904","title":"Recombinant Human PDGF-AA Protein (Active) - PKSH032904","description":"\u003cp\u003eRecombinant Human PDGF-AA Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032904-10, PKSH032904-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PDGF-AA\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Platelet-derived growth factor subunit A; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; PDGFA; PDGF1\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cardiovascular; Cancer; Developmental Biology; immunology; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P04085\u003c\/p\u003e\n\n\u003cp\u003eAccession: P04085\u003c\/p\u003e\n\n\u003cp\u003eBackground: Platelet-derived growth factor subunit A (PDGFA); belongs to the PDGF\/VEGF growth factor family. PDGFA is a secreted protein; stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells; connective tissue cells; bone and cartilage cells; and some blood cells. It plays an essential role in the regulation of embryonic development; cell proliferation; cell migration; survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis; normal development of the gastrointestinal tract; normal development of Leydig cells and spermatogenesis; normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing; Signaling is modulated by the formation of heterodimers with PDGFB.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cell proliferation assay using Balb\/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20-160 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ser87-Thr 211\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 4mM HCl.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 14.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 16 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118150254771,"sku":"PKSH032904-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118150287539,"sku":"PKSH032904-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_933af6df-a331-4e6e-bba7-34cc19316771.png?v=1709637131","url":"https:\/\/afsbio.com\/products\/recombinant-human-pdgf-aa-protein-active-pksh032904","provider":"AFSBio Inc.","version":"1.0","type":"link"}