{"product_id":"recombinant-human-pdgf-bb-ept197","title":"Recombinant Human PDGF-BB - EPT197","description":"\u003cp\u003eRecombinant Human PDGF-BB\u003c\/p\u003e\n\n\u003cp\u003eSize: 10ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: EPT197-10\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF\/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: PDGFBB; PDGF-BB\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Platelet-Derived Growth Factor BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed.\u003c\/p\u003e\n\n\u003cp\u003eAccession: P01127\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 12.42 KDa\u003c\/p\u003e\n\n\u003cp\u003eApparent Molecular Weight: 14 KDa, reducing conditions\u003c\/p\u003e\n\n\u003cp\u003eStorage Condition: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003ePurity: Greater than 98% as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 0.02 ng\/µg (0.2 EU\/µg) as determined by LAL test.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Measured in a cell proliferation assay using BALB\/c 3T3 cells. The ED50 for this effect is 5-20 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eRedissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg\/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\n\n\u003cp\u003eDelivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"Default Title","offer_id":43211043602611,"sku":"EPT197-10","price":390.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_dfb9244c-3dcc-497a-82b9-e09ff67d3a3d.png?v=1767117894","url":"https:\/\/afsbio.com\/products\/recombinant-human-pdgf-bb-ept197","provider":"AFSBio Inc.","version":"1.0","type":"link"}