{"product_id":"recombinant-human-pkc-iota-prkci-protein-gst-tag-pksh030411","title":"Recombinant Human PKC iota\/PRKCI Protein (GST Tag) - PKSH030411","description":"\u003cp\u003eRecombinant Human PKC iota\/PRKCI Protein (GST Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030411-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PKC iota, PRKCI\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: DXS1179E, nPKC-iota, PKCI\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction,\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_002731.4\u003c\/p\u003e\n\n\u003cp\u003eBackground: Protein kinase C iota type, also known as Atypical protein kinase C-lambda\/iota, aPKC-lambda\/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which belongs to theprotein kinase superfamily, AGC Ser\/Thr protein kinase family and PKC subfamily. PRKCI contains oneAGC-kinase C-terminal domain, oneOPR domain, onephorbol-ester\/DAG-type zinc finger and oneprotein kinase domain. PRKCI is predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. It is highly expressed in non-small cell lung cancers. PRKCI is a calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. It may play a role in the secretory response to nutrients. PRKCI is involved in cell polarization processes and the formation of epithelial tight junctions. It is implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. PRKCI functions in both pro- and anti-apoptotic pathways. It functions in the RAC1\/ERK signaling required for transformed growth. PRKCI plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). PRKCI might be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 10-Val 596\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 88 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 93.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 100 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43155022414003,"sku":"PKSH030411-50","price":796.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_014de2b7-c749-4c5b-805c-b3370b6c4ee2.png?v=1710436719","url":"https:\/\/afsbio.com\/products\/recombinant-human-pkc-iota-prkci-protein-gst-tag-pksh030411","provider":"AFSBio Inc.","version":"1.0","type":"link"}