{"product_id":"recombinant-human-pkc-nu-prkd3-protein-gst-tag-pksh030402","title":"Recombinant Human PKC nu\/PRKD3 Protein (GST Tag) - PKSH030402","description":"\u003cp\u003eRecombinant Human PKC nu\/PRKD3 Protein (GST Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030402-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PKC nu, PRKD3\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: EPK2, nPKC-NU, PKC-NU, PKD3, PRKCN\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction,\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_005804.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Serine\/threonine-protein kinase D3, also known as Protein kinase C nu type, Protein kinase EPK2, PRKD3, EPK2 and PRKCN, is a cytoplasm and membrane protein which belongs to theprotein kinase superfamily, CAMK Ser\/Thr protein kinase family and PKD subfamily. PRKD3 \/ PRKCN contains onePH domain, twophorbol-ester\/DAG-type zinc fingers and oneprotein kinase domain. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PRKD3 \/ PRKCN converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. It is involved in resistance to oxidative stress. PRKD3 \/ PRKCN is activated by DAG and phorbol esters. Phorbol-ester\/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735 and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain. PRKD3 \/ PRKCN can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. PRKD3 \/ PRKCN can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phospholipase C gamma and the involvement of other PKC family members.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Pro 890\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10mM Reduced Glutathione, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 126.7 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 126.7 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43155022315699,"sku":"PKSH030402-50","price":796.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_c8728ae3-5e7e-4998-b5cd-c52b70fc9066.png?v=1710436714","url":"https:\/\/afsbio.com\/products\/recombinant-human-pkc-nu-prkd3-protein-gst-tag-pksh030402","provider":"AFSBio Inc.","version":"1.0","type":"link"}