{"product_id":"recombinant-human-pklr-protein-his-tag-pksh032984","title":"Recombinant Human PKLR Protein (His Tag) - PKSH032984","description":"\u003cp\u003eRecombinant Human PKLR Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032984-10, PKSH032984-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PKLR\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Pyruvate Kinase Isozymes R\/L; Pyruvate Kinase 1; R-Type\/L-Type Pyruvate Kinase; Red Cell\/Liver Pyruvate Kinase; PKLR; PK1; PKL\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P30613\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cardiovascular; Cancer; epigenetics and nuclear signal; metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P30613\u003c\/p\u003e\n\n\u003cp\u003eBackground: Pyruvate Kinase Isozymes R\/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Ser574\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT 0.2M NaCl, 10% glycerol, pH 8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 62.9 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 58 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43068787130547,"sku":"PKSH032984-10","price":385.0,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43068787163315,"sku":"PKSH032984-50","price":735.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_5e8c31d8-3def-4e65-bf79-400b58d274a5.png?v=1708597625","url":"https:\/\/afsbio.com\/products\/recombinant-human-pklr-protein-his-tag-pksh032984","provider":"AFSBio Inc.","version":"1.0","type":"link"}