{"product_id":"recombinant-human-plk1-plk-1-protein-his-tagactive-pksh030399","title":"Recombinant Human PLK1\/PLK-1 Protein (His Tag)(Active) - PKSH030399","description":"\u003cp\u003eRecombinant Human PLK1\/PLK-1 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030399-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PLK1; PLK-1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: PLK; STPK13\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cell biology; Cancer; epigenetics and nuclear signal\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_005021.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: Serine \/ threonine-protein kinase PLK1 \/ PLK-1, also known as polo-like kinase 1 (PLK-1) or serine \/ threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine \/ threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 \/ PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 \/ PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 \/ PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 \/ PLK-1 localizes to the centrosomes, kinetochores and central spindle. Serine \/ threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC \/ C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 \/ PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 \/ PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 \/ PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be 5 nmol\/min\/mg using casein as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Ser 603\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 7.4, 0.5mM EDTA, 0.5mM EGTA, 0.5mM PMSF, 25% glycerol\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 70.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 66 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104286671027,"sku":"PKSH030399-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_6949c029-646b-432b-a6b9-8ac6d0fa5b6f.png?v=1709293796","url":"https:\/\/afsbio.com\/products\/recombinant-human-plk1-plk-1-protein-his-tagactive-pksh030399","provider":"AFSBio Inc.","version":"1.0","type":"link"}