{"product_id":"recombinant-human-pomgnt1-protein-his-tag-pksh032917","title":"Recombinant Human POMGNT1 Protein (His Tag) - PKSH032917","description":"\u003cp\u003eRecombinant Human POMGNT1 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032917-10, PKSH032917-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: POMGNT1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Protein O-Linked-Mannose Beta-1; 2-N-Acetylglucosaminyltransferase 1; POMGnT1; UDP-GlcNAc:Alpha-D-Mannoside Beta-1; 2-N-Acetylglucosaminyltransferase I.2; POMGNT1; MGAT1.2\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q8WZA1\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Tags \u0026amp; Cell Markers\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q8WZA1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3).\u003c\/p\u003e\n\n\u003cp\u003eSequence: Leu59-Thr660\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 69.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 74 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43068764782771,"sku":"PKSH032917-10","price":312.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43068764815539,"sku":"PKSH032917-50","price":830.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_ee62df1b-6a5d-4176-b140-97c726f8da73.png?v=1708597447","url":"https:\/\/afsbio.com\/products\/recombinant-human-pomgnt1-protein-his-tag-pksh032917","provider":"AFSBio Inc.","version":"1.0","type":"link"}