{"product_id":"recombinant-human-prkd2-pkd2-protein-his-gst-tagactive-pksh030353","title":"Recombinant Human PRKD2\/PKD2 Protein (His \u0026 GST Tag)(Active) - PKSH030353","description":"\u003cp\u003eRecombinant Human PRKD2\/PKD2 Protein (His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH030353-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PRKD2; PKD2\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: HSPC187; nPKC-D2; PKD2\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_057541.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: Serine\/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to theprotein kinase superfamily, CAMK Ser\/Thr protein kinase family and PKD subfamily. PRKD2 \/ PKD2 is widely expressed. It contains onePH domain, twophorbol-ester\/DAG-type zinc fingers and oneprotein kinase domain. PRKD2 \/ PKD2 is activated by DAG and phorbol esters. Phorbol-ester\/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 \/ PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be \u0026gt; 30 nmol\/min\/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Leu 878\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 82 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 50mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 124 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 120 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104285491379,"sku":"PKSH030353-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_1064c58f-700a-48b1-aa33-8a66e91f6292.png?v=1709293769","url":"https:\/\/afsbio.com\/products\/recombinant-human-prkd2-pkd2-protein-his-gst-tagactive-pksh030353","provider":"AFSBio Inc.","version":"1.0","type":"link"}