{"product_id":"recombinant-human-prkg1-protein-his-tag-pksh032242","title":"Recombinant Human PRKG1 Protein (His Tag) - PKSH032242","description":"\u003cp\u003eRecombinant Human PRKG1 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032242-10, PKSH032242-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PRKG1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: cGMP-Dependent Protein Kinase 1; cGK 1; cGK1; cGMP-Dependent Protein Kinase I; cGKI; PRKG1; PRKG1B; PRKGR1A; PRKGR1B\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q13976\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q13976-2\u003c\/p\u003e\n\n\u003cp\u003eBackground: cGMP-Dependent Protein Kinase 1 (PRKG1) belongs to the protein kinase superfamily and AGC Ser\/Thr protein kinase family. PRKG1 contains one AGC-kinase C-terminal domain, two cyclic nucleotide-binding domains, and one protein kinase domain. PRKG1 is mainly expressed in the lung and placenta. PRKG1 acts as a key mediator of the nitric oxide (NO)\/cGMP signaling pathway. PRKG1 can phosphorylate many proteins that regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm, and nociception.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Gly2-Pro686\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 80 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 20% glycerol, pH8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 78.8 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 75 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43149112672435,"sku":"PKSH032242-10","price":312.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43149112705203,"sku":"PKSH032242-50","price":830.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_e416062e-ac6b-435d-91e7-8f083e6386ee.png?v=1710340846","url":"https:\/\/afsbio.com\/products\/recombinant-human-prkg1-protein-his-tag-pksh032242","provider":"AFSBio Inc.","version":"1.0","type":"link"}