{"product_id":"recombinant-human-r-spondin-1-rspo1-protein-his-tagactive-pksh031269","title":"Recombinant Human R-Spondin 1\/RSPO1 Protein (His Tag)(Active) - PKSH031269","description":"\u003cp\u003eRecombinant Human R-Spondin 1\/RSPO1 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031269-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: R-Spondin 1; RSPO1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof plate-specific spondin-1\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Developmental Biology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture; ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_001033722.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt\/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.\u003c\/p\u003e\n\n\u003cp\u003eActivity: 1. Immobilized human RSPO1 at 20 μg\/ml (100 μl\/well) can bind human LIMPII with a linear ranger of 32-800 ng\/ml. \u003cbr\u003e\n2. Immobilized human RSPO1 at 20 μg\/ml (100 μl\/well) can bind mouse CD36 with a linear ranger of 6. 4-800 ng\/ml. \u003cbr\u003e\n3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg\/mL in the presence of 5 ng\/mL recombinant mouse Wnt3a.\u003c\/p\u003e \n\n\u003cp\u003eSequence: Met 1-Ala 263\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 28.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 42 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104306692275,"sku":"PKSH031269-100","price":1369.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_b3684b49-24d7-4a0e-9cfd-39efe894c8aa.png?v=1709293931","url":"https:\/\/afsbio.com\/products\/recombinant-human-r-spondin-1-rspo1-protein-his-tagactive-pksh031269","provider":"AFSBio Inc.","version":"1.0","type":"link"}