{"product_id":"recombinant-human-r-spondin-1-rspo1-protein-rp00071","title":"Recombinant Human R-spondin-1\/RSPO1 Protein - RP00071","description":"\u003cp\u003eRecombinant Human R-spondin-1\/RSPO1 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00071-10, RP00071-20, RP00071-50, RP00071-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human R-spondin-1\/RSPO1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg31-Ala263) of human R-Spondin1 (Accession #NP_001033722.1) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: RSPO1, CRISTIN3, RSPO\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q2MKA7\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 284654\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE; ≥ 90 % as determined by HPLC.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: This protein is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.\u003c\/p\u003e \n\n\u003cp\u003eBio-Activity: 1.Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10ng\/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression.|2.The intestinal crypts of mice were cultured in organoid culture medium containing factor combinations (100 ng\/mL Noggin, Cat. RP01237 + 500 ng\/mL R-spindin-1, Cat. RP00071) derived from ABclonal for144 hours, intestinal organoids were formed. (Customer Feedback Data)|3.Recombinant Human R-Spondin 1 protein stimulated Wnt signal pathway with Wnt-3a protein in HEK293T cells. After 6 hours, the stimulation when adding 300 ng\/mL of R-Spondin-1 reached highest effect. Compared with only Wnt-3a stimulation, the Wnt signaling pathway was enhanced 3.1-fold after adding 300 ng\/mL R-Spondin 1.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: RISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198294909107,"sku":"RP00071-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198294941875,"sku":"RP00071-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198294974643,"sku":"RP00071-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198295007411,"sku":"RP00071-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_e531d58e-5c76-4e73-b9ee-bfd16dee15ca.png?v=1766960245","url":"https:\/\/afsbio.com\/products\/recombinant-human-r-spondin-1-rspo1-protein-rp00071","provider":"AFSBio Inc.","version":"1.0","type":"link"}