{"product_id":"recombinant-human-siglec-10-c-fc-avi-biotinylated-pksh033985","title":"Recombinant Human Siglec-10 (C-Fc-Avi) Biotinylated - PKSH033985","description":"\u003cp\u003eRecombinant Human Siglec-10 (C-Fc-Avi) Biotinylated\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033985-20, PKSH033985-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Siglec-10\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: SIGLEC10; MGC126774; PRO940; Siglec10; SLG2; sialic acid-binding Ig-like lectin 10; Siglec-10; siglec-like gene 2; Siglec-like protein 2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Tags \u0026amp; Cell Markers\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Biotinylated\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-Fc-Avi\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q96LC7\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q96LC7\u003c\/p\u003e\n\n\u003cp\u003eBackground: Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33\/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2, 3 or alpha 2, 6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized Anti-Human Siglec10 mAb at 1μg\/ml (100 μl\/well) can bind Biotinylated Human Siglec-10-Fc-Avi(Cat#PKSH033985). The ED50 of Biotinylated Human Siglec-10-Fc-Avi is 1. 45 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met17--Thr546\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 86.9 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 100-130 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43118166212787,"sku":"PKSH033985-20","price":478.8,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43118166245555,"sku":"PKSH033985-100","price":1293.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_16a0771a-20fe-456d-ace5-00f89ba0f732.png?v=1709637406","url":"https:\/\/afsbio.com\/products\/recombinant-human-siglec-10-c-fc-avi-biotinylated-pksh033985","provider":"AFSBio Inc.","version":"1.0","type":"link"}