{"product_id":"recombinant-human-smad3-protein-his-flag-tag-pksh032763","title":"Recombinant Human SMAD3 Protein (His \u0026 Flag Tag) - PKSH032763","description":"\u003cp\u003eRecombinant Human SMAD3 Protein (His \u0026amp; Flag Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH032763-10, PKSH032763-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: SMAD3\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P84022\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cancer; epigenetics and nuclear signal; metabolism; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-6His-Flag\u003c\/p\u003e\n\n\u003cp\u003eAccession: P84022\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin\/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3\/SMAD4 complex, activates transcription. It also can form a SMAD3\/SMAD4\/JUN\/FOS complex at the AP-1\/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ser2-Ser425\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH7.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 50.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 60 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43068894445747,"sku":"PKSH032763-10","price":155.4,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43068894478515,"sku":"PKSH032763-50","price":415.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_11778fc2-c5dc-45a2-ba0a-8b98f4c7209d.png?v=1708598635","url":"https:\/\/afsbio.com\/products\/recombinant-human-smad3-protein-his-flag-tag-pksh032763","provider":"AFSBio Inc.","version":"1.0","type":"link"}