{"product_id":"recombinant-human-tgf-beta-3-tgfb3-protein-his-tag-pdeh100271","title":"Recombinant Human TGF-beta 3\/TGFB3 protein (His tag) - PDEH100271","description":"\u003cp\u003eRecombinant Human TGF-beta 3\/TGFB3 protein (His tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PDEH100271-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: TGF-beta 3; TGFB3\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: ARVD; ARVD1; LDS5; RNHF; TGFbeta 3; transforming growth factor beta-3\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P10600\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Stem Cells; Signal Transduction; Cancer\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P10600\u003c\/p\u003e\n\n\u003cp\u003eBackground: TGF--beta 3 (transforming growth factor-beta 3) is a member of a TGF--beta superfamily subgroup that is defined by their structural and functional similarities. TGF-beta 3 and its closely related proteins, TGF-beta 1 and - beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial--mesenchymal transition. The non-redundant biological effects of TGF-- beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development. Human TGF--beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin--like convertase to generate a 220 aa latency--associated peptide (LAP) and a 112 aa mature TGF--beta 3. Mature human TGF-- beta 3 shows 100%, 99%, and 98% aa identity with mouse\/dog\/horse, rat, and pig TGF-- beta 3, respectively. TGF-beta 3 is secreted as a latent complex. This latent form of TGF-beta 3 is activated by integrins, thrombospondin-1, plasmin, and matrix metalloproteases. It can also be activated by extreme pH and reactive oxygen species. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine\/threonine kinase receptor. This receptor then phosphorylates and activates type I serine\/threonine kinase receptors, TGF-- beta RI or ALK--1, to modulate transcription through Smad phosphorylation. The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-- beta RIII and endoglin) and the subsequent initiation of Smad--dependent or -independent signaling pathways.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala 301-Ser 412\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 12.21 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 30 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43083538890931,"sku":"PDEH100271-100","price":165.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_955749ee-9ddc-425c-b410-0837e4a23831.png?v=1708944312","url":"https:\/\/afsbio.com\/products\/recombinant-human-tgf-beta-3-tgfb3-protein-his-tag-pdeh100271","provider":"AFSBio Inc.","version":"1.0","type":"link"}