{"product_id":"recombinant-human-tgfb3-protein-active-pksh033140","title":"Recombinant Human TGFB3 Protein (Active) - PKSH033140","description":"\u003cp\u003eRecombinant Human TGFB3 Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033140-10, PKSH033140-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: TGFB3\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Transforming growth factor beta-3; TGFB3; TGF-beta-3; Latency-associated peptide; LAP\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cardiovascular; Cancer; metabolism; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P10600\u003c\/p\u003e\n\n\u003cp\u003eAccession: P10600\u003c\/p\u003e\n\n\u003cp\u003eBackground: Transforming growth factor beta 3(TGFB3) is a member of a TGF -β superfamily which is defined by theirstructural and functional similarities. TGFB3 is secreted as a complex with LAP. This latent form of TGFB3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin -1, and a subset ofintegrins. It binds with high affinity to TGF- β RII, a type II serine\/threonine kinase receptor. TGFB3 is involved incell differentiation, embryogenesis and development.It is believed to regulate molecules involved in cellularadhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-β3, mammals develop a deformity known as a cleft palate.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 mouse T cells. The ED50 for this effect is 10-80 pg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ala301-Ser412(Tyr340Phe)\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH2.5\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.01 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 12.7 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 12-14 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43118154023091,"sku":"PKSH033140-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43118154121395,"sku":"PKSH033140-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_e8be13e2-98f3-40b5-8fbb-8dffe4405432.png?v=1709637183","url":"https:\/\/afsbio.com\/products\/recombinant-human-tgfb3-protein-active-pksh033140","provider":"AFSBio Inc.","version":"1.0","type":"link"}