{"product_id":"recombinant-human-tie2-cd202b-protein-his-fc-tagactive-pksh031473","title":"Recombinant Human Tie2\/CD202b Protein (His \u0026 Fc Tag)(Active) - PKSH031473","description":"\u003cp\u003eRecombinant Human Tie2\/CD202b Protein (His \u0026amp; Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 200μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031473-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Tie2; CD202b\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CD202B; TIE-2; TIE2; VMCM; VMCM1\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cardiovascular; Cancer; Developmental Biology; Stem cells\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His \u0026amp; Fc\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_000450.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized recombinant human Angiopoietin-2 at 10 μg\/ml (100 μl\/well) can bind Human Tie2 \/ Fc chimera with a range of 0.2-20 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Lys 745\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 108.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 125-135 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104313016499,"sku":"PKSH031473-200","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_8dc023b8-91c1-4a32-afc5-78195be3efb1.png?v=1709294086","url":"https:\/\/afsbio.com\/products\/recombinant-human-tie2-cd202b-protein-his-fc-tagactive-pksh031473","provider":"AFSBio Inc.","version":"1.0","type":"link"}