{"product_id":"recombinant-human-tnf-beta-tnfb-protein-active-pksh033166","title":"Recombinant Human TNF beta\/TNFB Protein (Active) - PKSH033166","description":"\u003cp\u003eRecombinant Human TNF beta\/TNFB Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033166-20, PKSH033166-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: TNF beta; TNFB\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Lymphotoxin-Alpha; LT-Alpha; TNF-Beta; Tumor Necrosis Factor Ligand Superfamily Member 1; LTA; TNFB; TNFSF1\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Signal Transduction; Cancer; immunology;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P01374\u003c\/p\u003e\n\n\u003cp\u003eAccession: P01374\u003c\/p\u003e\n\n\u003cp\u003eBackground: Tumor Necrosis Factor β (TNF-β) is a secreted protein belonging to the tumor necrosis factor family. TNF-β binds to TNFRSF1A\/TNFR1, TNFRSF1B\/TNFBR and TNFRSF14\/HVEM in homotrimeric form, binds to TNFRSF3\/LTBR in heterotrimeric form with LTB. TNF-β forms heterotrimers with lymphotoxin-beta, which anchors TNF-β to the cell surface. TNF-β mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-β is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 20-80 pg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Leu35-Leu205\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 18.8 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 15 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43118154711219,"sku":"PKSH033166-20","price":348.6,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43118154743987,"sku":"PKSH033166-100","price":1034.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_fbee7e6e-03a4-480c-8516-e0e8f7629ce8.png?v=1709637195","url":"https:\/\/afsbio.com\/products\/recombinant-human-tnf-beta-tnfb-protein-active-pksh033166","provider":"AFSBio Inc.","version":"1.0","type":"link"}