{"product_id":"recombinant-human-tnfrsf10b-dr5-trail-r2-cd262-protein-rp00282","title":"Recombinant Human TNFRSF10B\/DR5\/TRAIL-R2\/CD262 Protein - RP00282","description":"\u003cp\u003eRecombinant Human TNFRSF10B\/DR5\/TRAIL-R2\/CD262 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00282-10, RP00282-20, RP00282-50, RP00282-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human TNFRSF10B\/DR5\/TRAIL-R2\/CD262 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ile 56 - Glu 182) of human DR5 (Accession #NP_003833.3) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CD262, DR5, KILLER, KILLER\/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, TNFRSF10B\u003c\/p\u003e\n\n\u003cp\u003eSWISS: O14763-1\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 8795\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: This protein is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10\/TRAIL\/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.\u003c\/p\u003e \n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human TRAIL at 2 μg\/mL (100 μL\/well) can bind Recombinant Human DR5 with a linear range of 14-56 ng\/mL.|2.Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 0.38-1.5 pg\/mL in the presence of 20 ng\/mL Recombinant Human TRAIL\/TNFSF10.|3.Measured by its binding ability in a functional ELISA.Immobilized Human TRAIL \/ TNFSF10 Protein at 2μg\/mL (100 μL\/well) can bind Human CD262 Protein with a linear range of 0.5-14.1 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: ITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKE\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198305329331,"sku":"RP00282-10","price":168.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198305362099,"sku":"RP00282-20","price":189.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198305394867,"sku":"RP00282-50","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198305427635,"sku":"RP00282-100","price":336.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_c527fafa-f177-434c-858c-661bff60fffc.png?v=1766960383","url":"https:\/\/afsbio.com\/products\/recombinant-human-tnfrsf10b-dr5-trail-r2-cd262-protein-rp00282","provider":"AFSBio Inc.","version":"1.0","type":"link"}