{"product_id":"recombinant-human-tnfsf11-rankl-cd254-protein-rp00183","title":"Recombinant Human TNFSF11\/RANKL\/CD254 Protein - RP00183","description":"\u003cp\u003eRecombinant Human TNFSF11\/RANKL\/CD254 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00183-10, RP00183-20, RP00183-50, RP00183-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human TNFSF11\/RANKL\/CD254 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly136-Asp317) of human TRANCE\/RANK L\/TNFSF11 (Accession #NP_143026.1) fused with a 6×His tag at the N-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: TNFSF11, CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf\u003c\/p\u003e\n\n\u003cp\u003eSWISS: O14788\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 8600\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE; ≥ 90 % as determined by HPLC.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. TNFSF11 is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TNFSF11 was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT\/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy.\u003c\/p\u003e \n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF11 Protein at 2 μg\/mL (100 μL\/well) can bind Mouse TNFRSF11B with a linear range of 0.049-13.74 ng\/mL.|2.Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte\/macrophage cells. The ED50 for this effect is 10.85-43.42 ng\/mL, corresponding to a specific activity of 2.30×104~9.22×104 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: N-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: GSQHIRAEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198302052531,"sku":"RP00183-10","price":168.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198302085299,"sku":"RP00183-20","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198302118067,"sku":"RP00183-50","price":420.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198302150835,"sku":"RP00183-100","price":672.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_a164858b-1810-4613-90aa-7ba8e7078b12.png?v=1766960340","url":"https:\/\/afsbio.com\/products\/recombinant-human-tnfsf11-rankl-cd254-protein-rp00183","provider":"AFSBio Inc.","version":"1.0","type":"link"}