{"product_id":"recombinant-human-tnfsf14-light-hvem-l-cd258-protein-rp01336","title":"Recombinant Human TNFSF14\/LIGHT\/HVEM-L\/CD258 Protein - RP01336","description":"\u003cp\u003eRecombinant Human TNFSF14\/LIGHT\/HVEM-L\/CD258 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01336-10, RP01336-20, RP01336-50, RP01336-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human TNFSF14\/LIGHT\/HVEM-L\/CD258 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp74-Val240) of Human TNFSF14 (Accession #NP_003798.2) fused with a 6×His tag at the N-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: TNFSF14, CD258, HVEML, LIGHT, LTg\u003c\/p\u003e\n\n\u003cp\u003eSWISS: O43557\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 8740\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 \/ LIGHT \/ CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 \/ LIGHT \/ CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14 \/ LIGHT \/ CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4+and CD8+T cells. In addition, TNFSF14 \/ LIGHT \/ CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 \/ LIGHT \/ CD258 is being actively investigated as a possible basis for cancer treatment.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF14 at 2 μg\/mL (100 μL\/well) can bind Mouse HVEM with a linear range of 0.02-0.89 μg\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: N-his\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: DGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGAL VVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSS SRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198317879475,"sku":"RP01336-10","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198317912243,"sku":"RP01336-20","price":266.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198317945011,"sku":"RP01336-50","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198317977779,"sku":"RP01336-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_a3191c73-bc53-434f-a686-2837f7541510.png?v=1766960537","url":"https:\/\/afsbio.com\/products\/recombinant-human-tnfsf14-light-hvem-l-cd258-protein-rp01336","provider":"AFSBio Inc.","version":"1.0","type":"link"}