{"product_id":"recombinant-human-tnfsf5-cd40-ligand-cd154-protein-rp01733","title":"Recombinant Human TNFSF5\/CD40 ligand\/CD154 Protein - RP01733","description":"\u003cp\u003eRecombinant Human TNFSF5\/CD40 ligand\/CD154 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01733-10, RP01733-20, RP01733-50, RP01733-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Active Recombinant Human CD40-L\/CD40L\/TNFSF5\/TRAP\/CD40LG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly116-Leu261) of human CD40-L\/CD40L\/TNFSF5\/TRAP\/CD40LG(Accession #NP_000065.1) fused with and a 6×His tag at the N-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40LG\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P29965\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 959\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its binding ability in a functional ELISA.Immobilized Human CD40L (Catalog: RP01733) at 5 μg\/mL (100 μL\/well) can bind Human CD40 (Catalog: RP01218) with a linear range of 0.005-0.5 μg\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: N-6His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: GDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198347731123,"sku":"RP01733-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198347763891,"sku":"RP01733-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198347796659,"sku":"RP01733-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198347829427,"sku":"RP01733-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_d2d0f3d4-d6f4-48f4-9635-84739e3e7f57.png?v=1766960880","url":"https:\/\/afsbio.com\/products\/recombinant-human-tnfsf5-cd40-ligand-cd154-protein-rp01733","provider":"AFSBio Inc.","version":"1.0","type":"link"}