{"product_id":"recombinant-human-tumor-necrosis-factor-alpha-his-rhutnf-α-his-pr1126","title":"Recombinant Human Tumor Necrosis Factor-alpha,His (rHuTNF-α-His) - PR1126","description":"\u003cp\u003eRecombinant Human Tumor Necrosis Factor-alpha,His (rHuTNF-α-His)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR1126-10, PR1126-50, PR1126-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10µg, 50µg, 1.0mg (Contact us for the 1.0mg option)\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight:Approximately 17.5 kDa. a single, non-glycosylated, polypeptide chain containing 157 amino acids fragment (77-233) and having a molecular mass of 21.85 kDa with an amino-terminal hexahistidine tag.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;95% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Fully biologically active when compared to standard. The Specific Activity is ≥2.0 × 107 IU\/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.0.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL\u003cbr\u003e\nRDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL\u003cbr\u003e\nLSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD\u003cbr\u003e\nFAESGQVYFG IIAL\u003c\/p\u003e\n\n\n\u003cp\u003eEndotoxin: Less than 1EU\/mg of rHuTNF-α-his as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.   Made  in China\u003c\/p\u003e\n\n\u003cp\u003eTumor necrosis factor alpha (TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10µg","offer_id":42880589889715,"sku":"PR1126-10","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"50µg","offer_id":42880589922483,"sku":"PR1126-50","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b086ee07-f8f4-4d53-b239-1c5e06e4db43.jpg?v=1701981995","url":"https:\/\/afsbio.com\/products\/recombinant-human-tumor-necrosis-factor-alpha-his-rhutnf-%ce%b1-his-pr1126","provider":"AFSBio Inc.","version":"1.0","type":"link"}