{"product_id":"recombinant-human-uba5-protein-his-tag-pksh033194","title":"Recombinant Human UBA5 Protein (His Tag) - PKSH033194","description":"\u003cp\u003eRecombinant Human UBA5 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH033194-10, PKSH033194-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: UBA5\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Ubiquitin-like modifier-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1; UBE1DC1; UBA5\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cell biology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-6His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q9GZZ9\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q9GZZ9\u003c\/p\u003e\n\n\u003cp\u003eBackground: UBA5 is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Met404\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 10% glycerol, pH 8.0 .\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 47.0 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 14\u0026amp;40-60 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43113925836979,"sku":"PKSH033194-10","price":385.0,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43113925869747,"sku":"PKSH033194-50","price":735.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_771336dd-7fb8-4fb8-99f1-e10a40bc8e91.png?v=1709548793","url":"https:\/\/afsbio.com\/products\/recombinant-human-uba5-protein-his-tag-pksh033194","provider":"AFSBio Inc.","version":"1.0","type":"link"}