{"product_id":"recombinant-human-ubiquitin-protein-rp01960","title":"Recombinant Human Ubiquitin protein - RP01960","description":"\u003cp\u003eRecombinant Human Ubiquitin protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20ug, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01960-20, RP01960-50, RP01960-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human Ubiquitin protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Gly76) of Human Ubiquitin (Accession #NP_001268645.1) fused with a His tag at the N-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: UBB, Polyubiquitin-B, Cleaved into: Ubiquitin\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P0CG47\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 7314\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: UBB is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin. In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes. Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.\u003c\/p\u003e\n\n\u003cp\u003eSource: E. coli\u003c\/p\u003e\n\n\u003cp\u003eTag: N-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"20ug","offer_id":45198378172595,"sku":"RP01960-20","price":182.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198378205363,"sku":"RP01960-50","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198378238131,"sku":"RP01960-100","price":224.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_16cd7ce1-469d-4ba7-af35-42ecfd88d3ec.png?v=1766961448","url":"https:\/\/afsbio.com\/products\/recombinant-human-ubiquitin-protein-rp01960","provider":"AFSBio Inc.","version":"1.0","type":"link"}