{"product_id":"recombinant-human-ve-cadherin-cdh5-protein-his-fc-tagactive-pksh031610","title":"Recombinant Human VE-Cadherin\/CDH5 Protein (His \u0026 Fc Tag)(Active) - PKSH031610","description":"Recombinant Human VE-Cadherin\/CDH5 Protein (His \u0026amp; Fc Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSH031610-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: VE-Cadherin; CDH5\n\nTarget Symptom: 7B4; CD144\n\nResearch Area: Signal Transduction; Tags \u0026amp; Cell Markers; Cardiovascular; Cancer; \n\nTarget Species: Human\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture; ELISA\n\nFusion Tag: C-His \u0026amp; Fc\n\nAccession: NP_001786.2\n\nBackground: Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based diagnoses, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.\n\nActivity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 10\u003csup\u003e4\u003c\/sup\u003e cells\/well are added to Recombinant Human Cadherin-5 coated plates (0.8 μg\/mL with 100 μL\/well), approximately \u0026gt; 50% will adhere after 1 hour at 37℃.\n\nSequence: Met 1-Gln 593\n\nPurity: \u0026gt; 85 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 92 kDa\n\nObserved MW: 120 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104318750899,"sku":"PKSH031610-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_4293fea3-424d-464b-803a-55ed8157972e.png?v=1709294197","url":"https:\/\/afsbio.com\/products\/recombinant-human-ve-cadherin-cdh5-protein-his-fc-tagactive-pksh031610","provider":"AFSBio Inc.","version":"1.0","type":"link"}