{"product_id":"recombinant-human-vegf-a-vegf165-protein-rp01150","title":"Recombinant Human VEGF-A\/VEGF165 Protein - RP01150","description":"\u003cp\u003eRecombinant Human VEGF-A\/VEGF165 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20ug, 50ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01150-10, RP01150-20, RP01150-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human VEGF-A\/VEGF165 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala27-Arg191) of human VEGF165 (Accession #NP_001165097.1) fused with a 6×His tag at the N-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: VEGFA, MVCD1, VEGF, VPF, vascular endothelial growth factor A, MVCD1, VEGF, VPF, L VEGFA, VEGF A\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P15692-4\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 7422\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGF165 at 1 μg\/mL (100 μL\/well) can bind Recombinant Human VEGFR2 with a linear range of 8-20 ng\/mL.|2.Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 2 μg\/mL (100 μL\/well) can bind Human KDR with a linear range of 0.2-11.6 ng\/mL.|3.Recombinant Human VEGF165 stimulates cell proliferation of the human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.19-0.78 ng\/mL, corresponding to a specific activity of 1.28 × 106~5.26× 106 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: N-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198366146739,"sku":"RP01150-10","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198366179507,"sku":"RP01150-20","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198366212275,"sku":"RP01150-50","price":280.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_2c77f16c-e12b-4ae9-a9e3-c1c0ea64220a.png?v=1766961253","url":"https:\/\/afsbio.com\/products\/recombinant-human-vegf-a-vegf165-protein-rp01150","provider":"AFSBio Inc.","version":"1.0","type":"link"}