{"product_id":"recombinant-human-vrk1-protein-his-gst-tag-pksh031091","title":"Recombinant Human VRK1 Protein (His \u0026 GST Tag) - PKSH031091","description":"\u003cp\u003eRecombinant Human VRK1 Protein (His \u0026amp; GST Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSH031091-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: VRK1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: PCH1; PCH1A\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q99986\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction; Cell biology; epigenetics and nuclear signal;\u003c\/p\u003e \n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His \u0026amp; GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q99986\u003c\/p\u003e\n\n\u003cp\u003eBackground: VRK1 is a member of the vaccinia-related kinase (VRK) family of serine\/threonine protein kinases. Serine\/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members; thereby playing a role in various processes such as cell metabolism; cell polarity; apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells; such as those in testis; thymus; fetal liver; and carcinomas. As a serine\/threonine kinase; VRK1 phosphorylates 'Thr-18' of p53\/TP53 and may thereby prevent the interaction between p53\/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1); also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem; central and peripheral motor dysfunction from birth; gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Lys 396\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 88 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 73 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 65-70 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43083645911219,"sku":"PKSH031091-50","price":680.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_b2661d0e-b8f8-4c85-abf4-df5981076e7e.png?v=1708946057","url":"https:\/\/afsbio.com\/products\/recombinant-human-vrk1-protein-his-gst-tag-pksh031091","provider":"AFSBio Inc.","version":"1.0","type":"link"}