{"product_id":"recombinant-human-xiap-birc4-protein-avi-tagactive-pksh031510","title":"Recombinant Human XIAP\/BIRC4 Protein (AVI Tag)(Active) - PKSH031510","description":"\u003cp\u003eRecombinant Human XIAP\/BIRC4 Protein (AVI Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSH031510-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: XIAP; BIRC4\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: API3; BIRC4; hIAP-3; hIAP3; IAP-3; ILP1; MIHA; XLP2\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Cell biology; Cancer; metabolism\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Biotinylated\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-Avi\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_001158.2\u003c\/p\u003e\n\n\u003cp\u003eBackground: E3 ubiquitin-protein ligase XIAP \/ BIRC4, also known as inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein, and IAP-like protein, is a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. XIAP \/ BIRC4 functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. XIAP \/ BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this encoding gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Thought to be the most potent apoptosis suppressor, XIAP \/ BIRC4, directly binds and inhibits caspases -3, -7 and -9. Survivin, which also binds to several caspases, is up-regulated in a many tumour cell types. Defects in XIAP \/ BIRC4 are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2). XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human SMAC-His at 10 μg\/ml (100 μl\/well) can bind recombinant human XIAP-AVI with a linear range of 0.125-1.0 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Leu 121-Thr 356\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 75 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 25mM Tris, 10mM DTT, 1% glycerol, 0.2M Glutamine Potassium, pH 8.0\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 29.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 29.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104315179187,"sku":"PKSH031510-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_0b89b57f-d269-4d09-83f6-40b22669867f.png?v=1709294122","url":"https:\/\/afsbio.com\/products\/recombinant-human-xiap-birc4-protein-avi-tagactive-pksh031510","provider":"AFSBio Inc.","version":"1.0","type":"link"}