{"product_id":"recombinant-human5-nucleotidase-nt5e-cd73-protein-rp00160","title":"Recombinant Human5'-Nucleotidase\/NT5E\/CD73 Protein - RP00160","description":"\u003cp\u003eRecombinant Human5'-Nucleotidase\/NT5E\/CD73 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP00160-10, RP00160-20, RP00160-50, RP00160-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human5'-Nucleotidase\/NT5E\/CD73 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Lys547) of human 5'-Nucleotidase\/CD73 (Accession #NP_002517.1) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: NT5E, CALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P21589\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 4907\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE; ≥ 90 % as determined by HPLC.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: 73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The proteins are used as determinants of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human 5-Nucleotidase\/CD73 at 2 μg\/mL (100 μL\/well) can bind 5-Nucleotidase\/CD73 Rabbit pAb with a linear range of 0.16-2.93 ng\/mL.|2.Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is \u0026gt;33654 pmol\/min\/μg.|3.Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is \u0026gt;25,000 pmol\/min\/μg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: MCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADETFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198300086451,"sku":"RP00160-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198300119219,"sku":"RP00160-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198300151987,"sku":"RP00160-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198300184755,"sku":"RP00160-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_48b7f9cd-a3a0-46d7-972f-0af6bb9a6ed3.png?v=1766960315","url":"https:\/\/afsbio.com\/products\/recombinant-human5-nucleotidase-nt5e-cd73-protein-rp00160","provider":"AFSBio Inc.","version":"1.0","type":"link"}