{"product_id":"recombinant-i-309-ccl1-human-bk0077","title":"Recombinant I-309\/CCL1, Human - BK0077","description":"\u003cp\u003eRecombinant I-309\/CCL1, Human\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0077-5, BK0077-25\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5μg, 25μg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 8.5 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% as analyzed by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: The EC50 value of human I-309\/CCL on Caˆ2+ mobilization assay in CHO-K1\/Gα15\/hCCR8 cells (human Gα15 and human CCR8 stably expressed in CHO-K1 cells) is less than 1 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against PBS.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: SKS MQVPFSRCCF SFAEQEIPLR AILCYRNTSS ICSNEGLIFK LKRGKEACAL Dt VGWVQRHR KMLRHCPSKR K\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant human I-309\/CCL1 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant human I-309\/CCL1 should be stable up to 1 week at 4°C or up to 2 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Chemokine (C-C motif) ligand 1 (CCL1), also known as I-309, is a small glycoprotein secreted by activated T cells that belongs to the family ofchemokines. Human CCL1 has been assumed to be a homologue of mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. CCL1 attracts monocytes, NK cells, immature B cells and dendritic cells by interacting with the cell surface chemokine receptor CCR8. This chemokine resides in a large cluster of CC chemokines on human chromosome 17.Recombinant Human I-309\/CCL1 produced in E.coli is a single non-glycosylated polypeptide chain containing 74 amino acids. A fully biologically active molecule, rhI-309\/CCL1has a molecular mass of 8.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"5μg","offer_id":42882240643251,"sku":"BK0077-5","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"25μg","offer_id":42882240676019,"sku":"BK0077-25","price":691.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f4eb7a35-0037-4d96-a84d-3f2cea601257.jpg?v=1702049866","url":"https:\/\/afsbio.com\/products\/recombinant-i-309-ccl1-human-bk0077","provider":"AFSBio Inc.","version":"1.0","type":"link"}