{"product_id":"recombinant-il-1β-porcine-bk0096","title":"Recombinant IL-1β, Porcine - BK0096","description":"\u003cp\u003eRecombinant IL-1β, Porcine\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0096-10, BK0096-50\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 17.7 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% as analyzed by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: ED50 \u0026lt; 1ng\/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 10ˆ6 IU\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against PBS.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MANVQSMEC KLQDKDHKSL VLAGPHMLKA LHLLTGDLKR EVVFCMSFVQ GDDSNNKIPV TLGIKGKNLY LSCVMKDNTP TLQLEDIDPK RYPKRDMEKR FVFYKTEIKN RVEFESALYP NWYISTSQAE QKPVFLGNSK GRQDITDFTM EVLSP\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant Porcine IL-1β, remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Porcine IL-1β should be stable up to 1 week at 4°C or up to 3 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Interleukin-1 beta (rpIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1α and IL-1β are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. While IL-1α and IL-1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity.Recombinant Porcine IL-1β produced in E.coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rpIL-1β has a molecular mass of 17.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882242117811,"sku":"BK0096-10","price":256.94,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882242150579,"sku":"BK0096-50","price":813.32,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ea74994c-6572-43f1-afaa-b0397cfd5db8.jpg?v=1702049909","url":"https:\/\/afsbio.com\/products\/recombinant-il-1%ce%b2-porcine-bk0096","provider":"AFSBio Inc.","version":"1.0","type":"link"}