{"product_id":"recombinant-il-4-mouse-bk0246","title":"Recombinant IL-4, Mouse - BK0246","description":"\u003cp\u003eRecombinant IL-4, Mouse\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0246-10, BK0246-50\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eSource: CHO\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 15 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% as analyzed by SDS-PAGE and HPLC.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: ED50 \u0026lt; 2 ng\/ml, measured in a cell proliferation assay using murine HT-2 cells, corresponding to a specific activity of \u0026gt;5 x 10ˆ5 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against PBS.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: HIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQR\u003cbr\u003e\n LFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant Murine Interleukin 4 (IL-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-4 should be stable up to 1 week at 4°C or up to 2 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells[1]. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity[2]. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells[3]. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure[4]. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet[5].\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882256371891,"sku":"BK0246-10","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882256404659,"sku":"BK0246-50","price":691.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_1c74532a-b2b4-4b97-acf9-9f68c6ff0ab6.jpg?v=1702050150","url":"https:\/\/afsbio.com\/products\/recombinant-il-4-mouse-bk0246","provider":"AFSBio Inc.","version":"1.0","type":"link"}