{"product_id":"recombinant-ld78-β-ccl3l1-human-bk0119","title":"Recombinant LD78-β\/CCL3L1, Human - BK0119","description":"\u003cp\u003eRecombinant LD78-β\/CCL3L1, Human\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0119-5, BK0119-25\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5μg, 25μg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 7.8 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% by SDS-PAGE and HPLC analysis.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: ED50\u0026lt; 0.4 µg\/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL3L1, corresponding to a specific activity of \u0026gt; 2.5×10ˆ3 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against PBS.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: APL AADTPTACCF SYTSRQIPQN FIADYFETSS QCSKPSVIFL TKRGRQVCAD PSEEWVQKYV SDLELSA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O at 100 μg\/mL.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant human LD78-beta\/CCL3L1 (rhLD78-beta) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhLD78-beta remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: LD78-beta\/CCL3L1 is a proinflammatory chemokine and the isoform of Macrophage Inflammatory Protein-1 alpha (MIP-1 alpha). LD78-beta is secreted by most mature leukocytes, predominantly macrophages, and its major receptor is the G-protein coupled receptor CCR5, which is also the co-receptor used by the HIV-1 virus for cell entry. LD78-beta has superior antiviral activity and induces a variety of immune cells, particularly CD8+ T cells and immature dendritic cells. LD78-beta attracts lymphocytes and macrophages to sites of inflammation and infection, and its functions are inhibited by Interleukin-4, Interleukin-10, and Interleukin-13. Importantly, the copy number variation of LD78-beta is associated with HIV susceptibility, indicating LD78-beta’s critical role in the disease.Recombinant human LD78-beta\/CCL3L1 (rhLD78-beta) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhLD78-beta has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"5μg","offer_id":42882243985587,"sku":"BK0119-5","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"25μg","offer_id":42882244018355,"sku":"BK0119-25","price":444.71,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_800c1eea-3679-471d-8bec-9e7c6059d0d4.jpg?v=1702049946","url":"https:\/\/afsbio.com\/products\/recombinant-ld78-%ce%b2-ccl3l1-human-bk0119","provider":"AFSBio Inc.","version":"1.0","type":"link"}