{"product_id":"recombinant-mip-1α-ccl3-human-bk0136","title":"Recombinant MIP-1α\/CCL3, Human - BK0136","description":"Recombinant MIP-1α\/CCL3, Human\n\nCatalogue Numbers: BK0136-10, BK0136-50\n\nSizes: 10μg, 50μg\n\nSource: Escherichia coli\n\nMolecular Weight: 7.8 kDa, observed by reducing SDS-PAGE.\n\nPurity: \u0026gt; 95% as analyzed by SDS-PAGE and HPLC.\n\nBiological Activity: ED50 \u0026lt; 80 ng\/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL3, corresponding to a specific activity of \u0026gt; 1.25×10ˆ4 units\/mg.\n\nPhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\n\nFormulation: Lyophilized after extensive dialysis against PBS.\n\nAA Sequence: ASL AADTPTACCF SYTSRQIPQN FIADYFETSS QCSKPGVIFL TKRSRQVCAD PSEEWVQKYV SDLELSA\n\nEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\n\nReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\n\nStorage: Lyophilized recombinant human MIP-1 alpha\/CCL3 (rhMIP-1 alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMIP-1 alpha remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.\n\nUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\n\nDescription: MIP-1 alpha\/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1α belongs to the CCL chemokine family, and shares 68% homology with MIP-1β. The mature form of MIP-1α contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1αin vivo\u003ci\u003e\u003c\/i\u003e are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1β, Interferon-γ, and lipoteichoic acid from Gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1α. MIP-1α augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. In addition, in wounds, MIP-1α chemo-attracts macrophages in order to accelerate the tissue repair process.Recombinant human MIP-1 alpha\/CCL3 (rhMIP-1 alpha) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-1 alpha has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882245001395,"sku":"BK0136-10","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882245034163,"sku":"BK0136-50","price":691.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a7925532-005b-4b5a-b614-4b49fe0815be.jpg?v=1702049967","url":"https:\/\/afsbio.com\/products\/recombinant-mip-1%ce%b1-ccl3-human-bk0136","provider":"AFSBio Inc.","version":"1.0","type":"link"}