{"product_id":"recombinant-mip-1α-ccl3-mouse-bk0322","title":"Recombinant MIP-1α\/CCL3, Mouse - BK0322","description":"\u003cp\u003eRecombinant MIP-1α\/CCL3, Mouse\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0322-5, BK0322-25\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5μg, 25μg\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK 293\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 7.8 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% as analyzed by SDS-PAGE and HPLC.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: The EC50 value of mouse MIP-1α\/CCL3 on Caˆ2+ mobilization assay in CHO-K1\/Gα15\/mCCR1 cells (human Gα15 and mouse CCR1 stably expressed in CHO-K1 cells) is less than 100 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against PBS.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: APYGADTPTACCFSYSRKIPRQFIVDYFETSSLCSQPGVIFLTKRNRQICADSKETWVQEYITDLELNA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant Mouse MIP-1α\/CCL3 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution Mouse MIP-1α\/CCL3 should be stable up to 1 week at 4°C or up to 2 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: MIP-1 alpha\/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1α belongs to the CCL chemokine family, and shares 68% homology with MIP-1β. The mature form of MIP-1α contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1αin vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1β, Interferon-γ, and lipoteichoic acid from gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1α. MIP-1α augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. Additionally, in wounds, MIP-1αchemoattracts macrophages in order to accelerate the tissue repair process.Recombinant Mouse MIP-1 alpha\/CCL3 (rmMIP-1α) produced in HEK 293cells is a single polypeptide chain containing 69 amino acids. A fully biologically active molecule, rmMIP-1αhas a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"5μg","offer_id":42882263154867,"sku":"BK0322-5","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"25μg","offer_id":42882263187635,"sku":"BK0322-25","price":375.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2a35b739-a80e-4ade-980b-adab58465475.jpg?v=1702050310","url":"https:\/\/afsbio.com\/products\/recombinant-mip-1%ce%b1-ccl3-mouse-bk0322","provider":"AFSBio Inc.","version":"1.0","type":"link"}