{"product_id":"recombinant-mouse-β-ngf-ngfb-protein-aa-122-241active-pksm041189","title":"Recombinant Mouse β-NGF\/NGFB Protein (aa 122-241)(Active) - PKSM041189","description":"\u003cp\u003eRecombinant Mouse β-NGF\/NGFB Protein (aa 122-241)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSM041189-20, PKSM041189-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: β-NGF; NGFB\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Beta-nerve growth factor; Beta-NGF; Ngf\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P01139\u003c\/p\u003e\n\n\u003cp\u003eAccession: P01139\u003c\/p\u003e\n\n\u003cp\u003eBackground: NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells The ED50 for this effect is 0.3-1. 5 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ser122-Gly241\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 13.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 14 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43118126596275,"sku":"PKSM041189-20","price":348.6,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43118126629043,"sku":"PKSM041189-100","price":1034.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_7db3bf04-c009-44e2-b316-638550b10596.png?v=1709636722","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-%ce%b2-ngf-ngfb-protein-aa-122-241active-pksm041189","provider":"AFSBio Inc.","version":"1.0","type":"link"}