{"product_id":"recombinant-mouse-alcam-cd166-protein-his-fc-tagactive-pksm040924","title":"Recombinant Mouse ALCAM\/CD166 Protein (His \u0026 Fc Tag)(Active) - PKSM040924","description":"\u003cp\u003eRecombinant Mouse ALCAM\/CD166 Protein (His \u0026amp; Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040924-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ALCAM; CD166\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: AI853494; BEN; CD166; DM-GRASP; MuSC; SC1\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His-Fc\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_033785.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Activated leukocyte cell adhesion molecule (ALCAM)\/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM\/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and\/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized mouse CD6-His at 10 μg\/ml (100 μl\/well) can bind mouse ALCAM-Fc2h, The EC50 of mouse ALCAM-Fc2h is 0.12-0.28 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Lys 527\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 84.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 105-110 kDa \u0026amp; 210 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104278675635,"sku":"PKSM040924-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_a7b1ddee-d05f-4400-ac74-877b4a49319c.png?v=1709293612","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-alcam-cd166-protein-his-fc-tagactive-pksm040924","provider":"AFSBio Inc.","version":"1.0","type":"link"}