{"product_id":"recombinant-mouse-b7-dc-pd-l2-cd273-protein-his-tagactive-pksm040480","title":"Recombinant Mouse B7-DC\/PD-L2\/CD273 Protein (His Tag)(Active) - PKSM040480","description":"Recombinant Mouse B7-DC\/PD-L2\/CD273 Protein (His Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSM040480-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: B7-DC; PD-L2; CD273\n\nTarget Symptom: Programmed cell death 1 ligand 2; Pdcd1lg2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; B7-DC; CD273; Btdc; F730015O22Rik; PD-L2\n\nTarget Species: Mouse\n\nExpression Host: HEK293 Cells\n\nApplication: ELISA\n\nFusion Tag: C-His\n\nUNIProt ID: Q9WUL5\n\nAccession: Q9WUL5\n\nBackground: Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-\u0026amp; gamma; in monocytes, or LPS plus IFN-\u0026amp; gamma; in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28\/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC\/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1\/PD-1.\n\nActivity: Immobilized mouse PD-L2-his at 10 μg\/mL (100 μl\/well) can bind mouse PD1-Fc. The EC50 of mouse PD1-Fc is 1. 63 μg\/mL.\n\nSequence: Met 1-Arg 219\n\nPurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 24 kDa\n\nObserved MW: 35-45 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104357875891,"sku":"PKSM040480-100","price":938.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_240867d1-9f53-4145-b583-cb53a4309bff.png?v=1709295169","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-b7-dc-pd-l2-cd273-protein-his-tagactive-pksm040480","provider":"AFSBio Inc.","version":"1.0","type":"link"}