{"product_id":"recombinant-mouse-b7-h4-c-fc-ept204","title":"Recombinant Mouse B7-H4 (C-Fc) - EPT204","description":"\u003cp\u003eRecombinant Mouse B7-H4 (C-Fc)\u003c\/p\u003e\n\n\u003cp\u003eSize: 10ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: EPT204-10\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mouse V-set domain-containing T-cell activation inhibitor 1\/VTCN1\/B7-H4 is glycosylated member of the B7 family of immune co‑stimulatory proteins. B7-H4 consists of extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: V-set domain containing T-cell activation inhibitor 1; B7 homolog 4; Protein B7S1; B7-H4; VTCN1\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse B7 Homolog 4 is produced by our Mammalian expression system and the target gene encoding Phe29-Pro258 is expressed with a Fc tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAccession: AAH32925.1\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 52.2 KDa\u003c\/p\u003e\n\n\u003cp\u003eApparent Molecular Weight: 70-90 KDa, reducing conditions\u003c\/p\u003e\n\n\u003cp\u003eStorage Condition: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003ePurity: Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Immobilized Anti-Human B7-H4 mAb (Cat#NC054) at 2μg\/ml (100 μl\/well) can bind Mouse B7-H4-Fc* (Cat#CS04).*: Biotinylated by NHS-biotin prior to testing The ED50 of Mouse B7-H4-Fc* (Cat#CS04) is 4.11 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eRedissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg\/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\n\n\u003cp\u003eDelivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"Default Title","offer_id":43211044454579,"sku":"EPT204-10","price":236.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_7f9b1004-6b2f-4e54-bd6f-6c77ed892b47.png?v=1767117897","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-b7-h4-c-fc-ept204","provider":"AFSBio Inc.","version":"1.0","type":"link"}