{"product_id":"recombinant-mouse-b7-h5-gi24-vsir-protein-his-tag-pksm041125","title":"Recombinant Mouse B7-H5\/Gi24\/VSIR Protein (His Tag) - PKSM041125","description":"\u003cp\u003eRecombinant Mouse B7-H5\/Gi24\/VSIR Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSM041125-10, PKSM041125-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: B7-H5; Gi24; VSIR\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Platelet receptor Gi24; stress induced secreted protein 1; Dies1; VISTA; SISP1; B7-H5; PD-1H; GI24\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q9D659\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q9D659\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mouse Platelet receptor Gi24(VISTA) is a transmembrane glycoprotein with homology to B7like immune costimulatory molecules. Mature mouse Gi24 contains a 159 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 97 aa cytoplasmic domain. VISTA promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. It supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but it is also down-regulated following BMP-4 exposure. It binds to BMP-4 directly and also associates with the type I BMP receptor Activin RIB\/ALK-4. It is expressed on the surface of naive CD4+ T cells and regulatory T cells. It is up-regulated in vivo on activated monocytes and dendritic cells. VISTA inhibits CD4+ and CD8+ T cell proliferation and their production of IL-2 and IFN-γ. Its expression on tumor cells attenuates the antitumor immune response and enables more rapid tumor progression.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Phe33-Ala191\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 18.6 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 30-40 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43072203456691,"sku":"PKSM041125-10","price":163.8,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43072203489459,"sku":"PKSM041125-50","price":415.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_7be3d7c7-d3a7-4777-ae4a-8e81f62d8a29.png?v=1708683845","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-b7-h5-gi24-vsir-protein-his-tag-pksm041125","provider":"AFSBio Inc.","version":"1.0","type":"link"}