{"product_id":"recombinant-mouse-caspase-8-casp8-protein-his-tag-pdem100044","title":"Recombinant Mouse Caspase-8\/CASP8 protein (His tag) - PDEM100044","description":"\u003cp\u003eRecombinant Mouse Caspase-8\/CASP8 protein (His tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PDEM100044-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Caspase-8; CASP8\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: CASP8_MOUSE; Caspase-8; Casp8; EC:3.4.22.61\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: O89110\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cell biology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: O89110\u003c\/p\u003e\n\n\u003cp\u003eBackground: Caspase-8 (Cysteine-aspartic acid protease 8\/Casp8a; also named MCH5, FLICA and MACH alpha 1) is a 28 kDa member of the peptidase C14A family of enzymes. It is widely expressed and is considered an initiating caspase for the apoptotic cascade. Caspase-8 acts on a wide variety of substrates, including procaspases 3, 4, 6, 7, 9 and 10, c-FLIPL and procaspase-8 itself. Human procaspase-8a is a 54-56 kDa, 479 amino acid (aa) protein. It contains two N-terminal death domains (aa 1-177), followed by a catalytic site that utilizes His317Gly318 plus Cys360. Normally, it is an inactive, cytosolic monomer. But following death-domain (DD) containing receptor oligomerization, Caspase-8 is recruited to the death-inducing signaling complex (DISC) that forms around the death domains of the oligomerized receptor.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ser 219-Gly 376\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 17.27 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 20 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43083570217139,"sku":"PDEM100044-100","price":165.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_4978ca09-784e-4d7f-8348-e53f07b4fd49.png?v=1708944800","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-caspase-8-casp8-protein-his-tag-pdem100044","provider":"AFSBio Inc.","version":"1.0","type":"link"}