{"product_id":"recombinant-mouse-cd27-tnfrsf7-protein-his-fc-tagactive-pksm040860","title":"Recombinant Mouse CD27\/TNFRSF7 Protein (His \u0026 Fc Tag)(Active) - PKSM040860","description":"\u003cp\u003eRecombinant Mouse CD27\/TNFRSF7 Protein (His \u0026amp; Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040860-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD27; TNFRSF7\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: CD27 antigen; CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7; S152; Tnfrsf7; Tp55\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His-Fc\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_001028298.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized recombinant mouse CD27 at 2 μg\/ml (100 μl\/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Arg 182\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 94 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 46.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 60-65 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104274186419,"sku":"PKSM040860-100","price":1316.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_1954c1ce-b457-4fd4-bf8b-37e20124e108.png?v=1709293559","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-cd27-tnfrsf7-protein-his-fc-tagactive-pksm040860","provider":"AFSBio Inc.","version":"1.0","type":"link"}