{"product_id":"recombinant-mouse-csf-1-m-csf-protein-rp01216","title":"Recombinant Mouse CSF-1\/M-CSF Protein - RP01216","description":"\u003cp\u003eRecombinant Mouse CSF-1\/M-CSF Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01216-10, RP01216-20, RP01216-50, RP01216-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse CSF-1\/M-CSF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu262) of mouse M-CSF\/CSF-1 (Accession #NP_031804.3.) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: MCSF, M-CSF, CSF-1, Lanimostim, CSF1\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P07141-1\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 12977\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte \/ macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF\/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF\/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF\/CSF-1 may also be involved in development of the placenta. The active form of M-CSF\/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF\/CSF-1 induces cells of the monocyte\/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF\/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte\/macrophage lineage, but also by regulating endothelial cell chemokine expression.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured in a cell proliferation assay using mouse bone marrow cells. The ED50 for this effect is 7.5-30.1 ng\/mL, corresponding to a specific activity of 3.32×10^4 ~1.33×10^5 units\/mg.| 2.Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is 8.31-33.24 ng\/mL, corresponding to a specific activity of 3.01×10^4 ~1.20×10^5 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: MTARGAAGRCPSSTWLGSRLLLVCLLMSRSIAKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKPDCNCLYPKATPSSDPASASPHQPPAPSMAPLAGLAWDDSQRTEGSSLLPSELPLRIEDPGSAKQRPPRSTCQTLE\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.01 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198327677107,"sku":"RP01216-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198327709875,"sku":"RP01216-20","price":308.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198327742643,"sku":"RP01216-50","price":490.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198327775411,"sku":"RP01216-100","price":784.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_f35a997a-5d30-420e-bf50-f7676f4fb76c.png?v=1766960636","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-csf-1-m-csf-protein-rp01216","provider":"AFSBio Inc.","version":"1.0","type":"link"}