{"product_id":"recombinant-mouse-csnk2a1-ck2a1-protein-his-gst-tagactive-pksm040295","title":"Recombinant Mouse CSNK2A1\/CK2A1 Protein (His \u0026 GST Tag)(Active) - PKSM040295","description":"\u003cp\u003eRecombinant Mouse CSNK2A1\/CK2A1 Protein (His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040295-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CSNK2A1; CK2A1\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Csnk2a1-rs4\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His-GST\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q60737\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q60737\u003c\/p\u003e\n\n\u003cp\u003eBackground: Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser \/ Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine \/ threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine \/ threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via th phosphorylation of Akt.\u003c\/p\u003e\n\n\u003cp\u003eActivity: The specific activity was determined to be 34 nmol\/min\/mg using casein as substrate.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met1-Gln391\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 72.9 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 65 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104354238643,"sku":"PKSM040295-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_91cc21f4-edbf-4e4b-bccf-9d8ca2d0d6d3.png?v=1709295055","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-csnk2a1-ck2a1-protein-his-gst-tagactive-pksm040295","provider":"AFSBio Inc.","version":"1.0","type":"link"}